The effect of protocatechuic acid on neuropathic pain and possible mechanism

The effect of protocatechuic acid on neuropathic pain and possible mechanism

Protocatechuic acid is a phenolic antioxidant whose effect on neuropathic pain has not been reported before, which is prominent by its effect on the progression of neurodegenerative diseases.

The aim of this study was to determine the possible effect of protocatechuic acid on neuropathic pain and to evaluate its effectiveness by clarifying its relationship with KATP channels and A1R stimulation.

A model of neuropathic pain induced by chronic construction nerve injury in rats was established and the anti-allodynic effect of 75, 150 and 300 mg/kg protocatechuic acid was evaluated with electronic von Frey device, the antihyperalgesic effect was evaluated with plantar test and the effect was compared with 50 and 100 mg/kg carbamazepine effect. The advantage of a combination of 150 mg/kg protocatechuic acid and 50 mg/kg carbamazepine was investigated. The contribution of A1R stimulation and KATP channel opening to protocatechuic acid induced anti-allodynic and antihyperalgesic activity was investigated using A1R antagonist DPCPX (3 μg) and KATP channel antagonist glibenclamide (60.7 nmol), respectively. Application of 75, 150 and 300 mg/kg of protocatechuic acid showed anti-allodynic and antihyperalgesic activity, respectively, by increasing the mechanical and thermal thresholds associated with neuropathy development without changing locomotor activity.

The effect obtained from the combination of protocatechuic acid with carbamazepine was found to be more powerful than those obtained from all drug applications. The anti-allodynic and antihyperalgesic efficacy of protocatechuic acid was found to be a marked contribution with ATP channel activation accompanying A1R stimulation.

Although the mechanism of action should be elucidated in more detail, preclinical data were obtained to predict the profile of effect. Based on these data, protocatechuic acid is thought to have the potential to be used alone or as an adjuvant in the management of neuropathic pain. The results obtained from the thesis study provide data that will contribute to the new treatment approaches.

Keywords: Protocatechuic Acid, Mechanical Allodynia, Thermal Hyperalgesia, KATP Channels, A1 Adenosine Receptor

-This research is made by Melda Ozgurbuz

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